Congestion Avoidance Energy Efficient MAC Protocol for Wireless Sensor Networks

Wireless Sensor Network (WSNs) are generally energy-constrained and resource-constrained. When multiple simultaneous events occur in densely deployed WSNs, nodes near the base station can become congested, decreasing the network performance. Additionally, multiple nodes may sense an event leading to spatially-correlated contention, further increasing congestion. In order to mitigate the effects of congestion near the base station, an energy-efficient Media Access Control (MAC) protocol that can handle multiple simultaneous events and spatially-correlated contention is needed. Energy efficiency is important and can be achieved using duty cycles but they could degrade the network performance in terms of latency. Existing protocols either provide support for congestion near the base station or for managing spatially-correlated contention. To provide energy-efficiency while maintaining the networks performance under higher traffic load, we propose an energy-efficient congestion-aware MAC protocol. This protocol provides support for congestion near the base station and spatially-correlated contention by employing a traffic shaping approach to manage the arrival times of packets to the layers close to the base station. We implemented our protocol using the ns-2 simulator for evaluating its performance. Results show that our protocol has an improvement in the number of packets received at the base station while consuming less energy

Molecular Evolution of Broadly Neutralizing Llama Antibodies to the CD4-Binding Site of HIV-1

To date, no immunization of humans or animals has elicited broadly neutralizing sera able to prevent HIV-1 transmission; however, elicitation of broad and potent heavy chain only antibodies (HCAb) has previously been reported in llamas. In this study, the anti-HIV immune responses in immunized llamas were studied via deep sequencing analysis using broadly neutralizing monoclonal HCAbs as a guides. Distinct neutralizing antibody lineages were identified in each animal, including two defined by novel antibodies (as variable regions called VHH) identified by robotic screening of over 6000 clones. The combined application of five VHH against viruses from clades A, B, C and CRF_AG resulted in neutralization as potent as any of the VHH individually and a predicted 100% coverage with a median IC50 of 0.17 µg/ml for the panel of 60 viruses tested. Molecular analysis of the VHH repertoires of two sets of immunized animals showed that each neutralizing lineage was only observed following immunization, demonstrating that they were elicited de novo. Our results show that immunization can induce potent and broadly neutralizing antibodies in llamas with features similar to human antibodies and provide a framework to analyze the effectiveness of immunization protocols